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arXiv · 0708.3336

Far-field fluorescence microscopy beyond the diffraction limit: Fluorescence imaging with ultrahigh resolution

James H. Rice
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Abstract

Fluorescence microscopy is an important and extensively utilised tool for imaging biological systems. However, the image resolution that can be obtained has a limit as defined through the laws of diffraction. Demand for improved resolution has stimulated research into developing methods to image beyond the diffraction limit based on far-field fluorescence microscopy techniques. Rapid progress is being made in this area of science with methods emerging that enable fluorescence imaging in the far-field to possess a resolution well beyond the diffraction limit. This review outlines developments in far-field fluorescence methods which enable ultrahigh resolution imaging and application of these techniques to biology. Future possible trends and directions in far-field fluorescence imaging with ultrahigh resolution are also outlined.

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James H. Rice. 2007-08-24. Far-field fluorescence microscopy beyond the diffraction limit: Fluorescence imaging with ultrahigh resolution. https://arxiv.org/abs/0708.3336

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