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arXiv · 0807.2756

Fast fluorescence dynamics in non-ratiometric calcium indicators

M. Gersbach·D. L. Boiko·C. Niclass·C. Petersen·E. Charbon
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Abstract

A fluorescence decay of high-affinity non-ratiometric Ca2+ indicator Oregon Green BAPTA-1 (OGB-1) is analyzed with unprecedented temporal resolution in the two-photon excitation regime. A triple exponential decay is shown to best fit the fluorescence dynamics of OGB-1. We provide a new model for accurate measurements of the free Ca2+ concentration and dissociation constants of non-ratiometric calcium indicators.

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M. Gersbach, D. L. Boiko, C. Niclass, C. Petersen, E. Charbon. 2008-11-13. Fast fluorescence dynamics in non-ratiometric calcium indicators. https://doi.org/10.1364/ol.34.000362

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