A novel approach for structure analysis of two-dimensional membrane protein crystals using x-ray powder diffraction data

Ruben A. Dilanian; Connie Darmanin; Jose N. Varghese; Steve W. Wilkins; Toshihiko Oka; Naoto Yagi; Harry M. Quiney; Keith A. Nugent

arXiv · 1007.0065

A novel approach for structure analysis of two-dimensional membrane protein crystals using x-ray powder diffraction data

Ruben A. Dilanian·Connie Darmanin·Jose N. Varghese·Steve W. Wilkins·Toshihiko Oka·Naoto Yagi·Harry M. Quiney·Keith A. Nugent

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Abstract

The application of powder diffraction methods in two-dimensional crystallography is regarded as intractable because of the uncertainties associated with overlapping reflections. Here, we report an approach that resolves these ambiguities and provides reliable low-resolution phase information directly from powder diffraction data. We apply our method to the recovery of the structure of the bacteriorhodopsin (bR) molecule to a resolution of 7 angstroms using only powder diffraction data obtained from two-dimensional purple membrane (PM) crystals.

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Ruben A. Dilanian, Connie Darmanin, Jose N. Varghese, Steve W. Wilkins, Toshihiko Oka, Naoto Yagi, Harry M. Quiney, Keith A. Nugent. 2010-07-01. A novel approach for structure analysis of two-dimensional membrane protein crystals using x-ray powder diffraction data. https://arxiv.org/abs/1007.0065

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