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arXiv · 1708.04693

Structured illumination microscopy for dual-modality 3D sub-diffraction resolution fluorescence and refractive-index reconstruction

Shwetadwip Chowdhury·Will J. Eldridge·Adam Wax·Joseph A. Izatt
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Abstract

Though structured illumination (SI) microscopy is a popular imaging technique conventionally associated with fluorescent super-resolution, recent works have suggested its applicability towards sub-diffraction coherent imaging with quantitative endogenous biological contrast. Here, we demonstrate that SI can efficiently integrate the principles of fluorescent super-resolution and coherent synthetic aperture to achieve 3D dual-modality sub-diffraction resolution, fluorescence and refractive-index (RI) visualizations of biological samples. Such a demonstration can enable future biological studies to synergistically explore molecular and biophysical/biochemical mechanisms at sub-diffraction resolutions. We experimentally demonstrate this framework by introducing a SI microscope capable of 3D sub-diffraction fluorescence and RI imaging, and verify its biological visualization capabilities by experimentally reconstructing 3D RI/fluorescence visualizations of fluorescent calibration microspheres as well as alveolar basal epithelial adenocarcinoma (A549) and human colorectal adenocarcinmoa (HT-29) cells, fluorescently stained for F-actin.

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Shwetadwip Chowdhury, Will J. Eldridge, Adam Wax, Joseph A. Izatt. 2017-08-15. Structured illumination microscopy for dual-modality 3D sub-diffraction resolution fluorescence and refractive-index reconstruction. https://doi.org/10.1364/boe.8.005776

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