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arXiv · 1810.13018

Wavelength agile multi-photon microscopy with a fiber amplified diode laser

Matthias Eibl·Daniel Weng·Hubertus Hakert·Jan Philip Kolb·Tom Pfeiffer·Jennifer E. Hundt·Robert Huber·Sebastian Karpf
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Abstract

Multi-photon microscopy is a powerful tool in biomolecular research. Less complex and more cost effective excitation light sources will make this technique accessible to a broader community. Especially semiconductor diode seeded fiber lasers have proven to be robust, low cost and easy to use. However, their wavelength tuning range is often limited, so only a limited number of fluorophores can be accessed. Therefore, different approaches have been proposed to extend the spectral coverage of these lasers. Recently, we showed that four-wave mixing (FWM) assisted stimulated Raman scattering (SRS) can be harnessed to red-shift high power pulses from 1064 nm to a narrowband output at 1122 nm and 1186 nm and therefore extend the number of accessible fluorophores. In this contribution, we show the applicability of all three wavelengths for multi-photon microscopy and analyze the performance.

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Matthias Eibl, Daniel Weng, Hubertus Hakert, Jan Philip Kolb, Tom Pfeiffer, Jennifer E. Hundt, Robert Huber, Sebastian Karpf. 2018-11-01. Wavelength agile multi-photon microscopy with a fiber amplified diode laser. https://arxiv.org/abs/1810.13018

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