Nonlinear analysis of the fluid-solid transition in a model for ordered biological tissues

Abstract

The rheology of biological tissues is important for their function, and we would like to better understand how single cells control global tissue properties such as tissue fluidity. A confluent tissue can fluidize when cells diffuse by executing a series of cell rearrangements, or T1 transitions. In a disordered 2D vertex model, the tissue fluidizes when the T1 energy barriers disappear as the target shape index approaches a critical value ($s^*_{0} \sim 3.81$), and the shear modulus describing the linear response also vanishes at this same critical point. However, the ordered ground states of 2D vertex models become linearly unstable at a lower value of the target shape index (3.72) [1,2]. We investigate whether the ground states of the 2D vertex model are fluid-like or solid-like between 3.72 and 3.81 $-$ does the "equation of state" for these systems have two branches, like glassy particulate matter, or only one? Using four-cell and many-cell numerical simulations, we demonstrate that for a hexagonal ground state, T1 energy barriers only vanish at $\sim 3.81$, indicating that ordered systems have the same critical point as disordered systems. We also develop a simple geometric argument that correctly predicts how non-linear stabilization disappears at $s^*_{0}$ in ordered systems.