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arXiv · 1910.06851

Jointly super-resolved and optically sectioned Bayesian reconstruction method for structured illumination microscopy

Yann Lai-Tim·Laurent M. Mugnier·François Orieux·Roberto Baena-Gallé·Michel Paques·Serge Meimon
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Abstract

Structured Illumination Microscopy (SIM) is an imaging technique for achieving both super-resolution (SR) and optical sectioning (OS) in wide-field microscopy. It consists in illuminating the sample with periodic patterns at different orientations and positions. The resulting images are then processed to reconstruct the observed object with SR and/or OS. In this work, we present BOSSA-SIM, a general-purpose SIM reconstruction method, applicable to moving objects such as encountered in in vivo retinal imaging, that enables SR and OS jointly in a fully unsupervised Bayesian framework. By modeling a 2-layer object composed of an in-focus layer and a defocused layer, we show that BOSSA-SIM is able to jointly reconstruct them so as to get a super-resolved and optically sectioned in-focus layer. The achieved performance, assessed quantitatively by simulations for several noise levels, compares favorably with a state-of-the-art method. Finally, we validate our method on open-access experimental microscopy data.

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Yann Lai-Tim, Laurent M. Mugnier, François Orieux, Roberto Baena-Gallé, Michel Paques, Serge Meimon. 2019-10-17. Jointly super-resolved and optically sectioned Bayesian reconstruction method for structured illumination microscopy. https://doi.org/10.1364/oe.27.033251

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