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arXiv · 2002.08425

Single DNA Electron Spin Resonance Spectroscopy in Aqueous Solutions

Fazhan Shi·Fei Kong·Pengju Zhao·Xiaojun Zhang·Ming Chen·Sanyou Chen·Qi Zhang·Mengqi Wang·Xiangyu Ye·Zhecheng Wang·Zhuoyang Qin·Xing Rong·Jihu Su·Pengfei Wang·Peter Z. Qin·Jiangfeng Du
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Abstract

Magnetic resonance spectroscopy of single biomolecules under near-physiological conditions may substantially advance understanding of biological function, yet remains very challenging. Here we use nitrogen-vacancy centers in diamonds to detect electron spin resonance spectra of individual, tethered DNA duplexes labeled with a nitroxide spin label in aqueous buffer solutions at ambient temperatures. This paves the way for magnetic resonance studies on single biomolecules and their inter-molecular interactions in a native-like environment.

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Fazhan Shi, Fei Kong, Pengju Zhao, Xiaojun Zhang, Ming Chen, Sanyou Chen, Qi Zhang, Mengqi Wang, Xiangyu Ye, Zhecheng Wang, Zhuoyang Qin, Xing Rong, Jihu Su, Pengfei Wang, Peter Z. Qin, Jiangfeng Du. 2020-02-19. Single DNA Electron Spin Resonance Spectroscopy in Aqueous Solutions. https://doi.org/10.1038/s41592-018-0084-1

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