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arXiv · 2004.01710

Characterizing Halloumi cheese bacterial communities through metagenomic analysis

Eleni Kamilari·Dimitrios A. Anagnostopoulos·Photis Papademas·Andreas Kamilaris·Dimitris Tsaltas
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Abstract

Halloumi is a semi hard cheese produced in Cyprus for centuries and its popularity has significantly risen over the past years. High throughput sequencing (HTS) was applied in the present research to characterize traditional Cyprus Halloumi bacterial diversity. Eighteen samples made by different milk mixtures and produced in different areas of the country were analyzed, to reveal that Halloumi microbiome was mainly comprised by lactic acid bacteria (LAB), including Lactobacillus, Leuconostoc, and Pediococcus, as well as halophilic bacteria, such as Marinilactibacillus and Halomonas. Additionally, spore forming bacteria and spoilage bacteria, were also detected. Halloumi produced with the traditional method, had significantly richer bacterial diversity compared to Halloumi produced with the industrial method. Variations detected among the bacterial communities highlight the contribution of the initial microbiome that existed in milk and survived pasteurization, as well as factors associated with Halloumi manufacturing conditions, in the final microbiota composition shaping. Identification and characterization of Halloumi microbiome provides an additional, useful tool to characterize its typicity and probably safeguard it from fraud products that may appear in the market. Also, it may assist producers to further improve its quality and guarantee consumers safety.

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Eleni Kamilari, Dimitrios A. Anagnostopoulos, Photis Papademas, Andreas Kamilaris, Dimitris Tsaltas. 2020-04-03. Characterizing Halloumi cheese bacterial communities through metagenomic analysis. https://doi.org/10.1016/j.lwt.2020.109298

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