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arXiv · 2103.08552

Effects of a mesoporous bioactive glass on osteoblasts, osteoclasts and macrophages

N. Gomez-Cerezo·L. Casarrubios·I. Morales·M. J. Feito·M. Vallet-Regi·D. Arcos·M. T. Portoles
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Abstract

A mesoporous bioactive glass (MBG) of molar composition 75SiO2-20CaO-5P2O5 (MBG-75S) has been synthetized as a potential bioceramic for bone regeneration purposes. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), nitrogen adsorption studies and transmission electron microscopy (TEM) demonstrated that MBG-75Spossess a highly ordered mesoporous structure with high surface area and porosity, which would explain the high ionic exchange rate (mainly calcium and silicon soluble species) with the surrounded media. MBG-75S showed high biocompatibility in contact with Saos-2 osteoblast-like cells. Concentrations up to 1 mg/ml did not lead to significant alterations on either morphology or cell cycle. Regarding the effects on osteoclasts, MBG-75S allowed the differentiation of RAW-264.7 macrophages into osteoclast-like cells but exhibiting a decreased resorptive activity. These results point out that MBG-75S does not inhibit osteoclastogenesis but reduces the osteoclast boneresorbing capability. Finally, in vitro studies focused on the innate immune response, evidenced that MBG-75S allows the proliferation of macrophages without inducing their polarization towards the M1 pro-inflammatory phenotype. This in vitro behavior is indicative that MBG-75S would just induce the required innate immune response without further inflammatory complications under in vivo conditions. The overall behavior respect to osteoblasts, osteoclasts and macrophages, makes this MBG a very interesting candidate for bone grafting applications in osteoporotic patients.

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N. Gomez-Cerezo, L. Casarrubios, I. Morales, M. J. Feito, M. Vallet-Regi, D. Arcos, M. T. Portoles. 2021-03-15. Effects of a mesoporous bioactive glass on osteoblasts, osteoclasts and macrophages. https://doi.org/10.1016/j.jcis.2018.05.099

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