Feedback Microrheology in Soft Matter

Abstract

Soft matter consists of meso-scale (nm~μm) structures that are formed by weak interactions and reorganized under thermal fluctuations. The resulting complex relaxation phenomena may be probed with microrheology, by observing the movement of embedded probe particles. Because of the softness of the material, however, perturbations to the probe that are inevitably added during microrheology experiments prevent direct translation of those movements to rheological properties. In this study, we conducted optical-trap-based microrheology with significantly reduced mechanical perturbations; dual feedback technology allowed us to apply well-determined optical-trapping forces to a fluctuating embedded probe and precisely measure its response and fluctuations with high spatiotemporal resolution. We demonstrate the improved performance of this technique by studying an reconstituted network of actin cytoskeletal filaments, observing their slow dynamics, homogeneous thermal fluctuations as well as activated hopping between mesoscale microenvironments.