Single-shot multispectral quantitative phase imaging using deep neural network

Abstract

Multi-spectral quantitative phase imaging (MS-QPI) is a cutting-edge label-free technique to determine the morphological changes, refractive index variations and spectroscopic information of the specimens. The bottleneck to implement this technique to extract quantitative information, is the need of more than two measurements for generating MS-QPI images. We propose a single-shot MS-QPI technique using highly spatially sensitive digital holographic microscope assisted with deep neural network (DNN). Our method first acquires the interferometric datasets corresponding to multiple wavelengths (λ=532, 633 and 808 nm used here). The acquired datasets are used to train generative adversarial network (GAN) to generate multi-spectral quantitative phase maps from a single input interferogram. The network is trained and validated on two different samples, the optical waveguide and a MG63 osteosarcoma cells. Further, validation of the framework is performed by comparing the predicted phase maps with experimentally acquired and processed multi-spectral phase maps. The current MS-QPI+DNN framework can further empower spectroscopic QPI to improve the chemical specificity without complex instrumentation and color-cross talk.