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arXiv · 2204.08249

Label-free metabolic imaging of non-alcoholic-fatty-liver-disease (NAFLD) liver by volumetric dynamic optical coherence tomography

Pradipta Mukherjee·Shinichi Fukuda·Donny Lukmanto·Toshiharu Yamashita·Kosuke Okada·Shuichi Makita·Ibrahim Abd El-Sadek·Arata Miyazawa·Lida Zhu·Rion Morishita·Antonia Lichtenegger·Tetsuro Oshika·Yoshiaki Yasuno
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Abstract

Label-free metabolic imaging of non-alcoholic fatty liver disease (NAFLD) mouse liver is demonstrated ex vivo by dynamic optical coherence tomography (OCT). The NAFLD mouse is a methionine choline-deficient (MCD)-diet model, and two mice fed MCD diet for 1 and 2 weeks are involved in addition to a normal-diet mouse. The dynamic OCT is based on repeating raster scan and logarithmic intensity variance (LIV) analysis which enables volumetric metabolic imaging with a standard-speed (50,000 A-lines/s) OCT system. Metabolic domains associated with lipid droplet accumulation and inflammation are clearly visualized three-dimensionally. Particularly, the normal-diet liver exhibits highly metabolic vessel-like structures of peri-vascular hepatic zones. The 1-week MCD-diet liver shows ring-shaped highly metabolic structures formed with lipid droplets. The 2-week MCD-diet liver exhibits fragmented vessel-like structures associated with inflammation. These results imply that volumetric LIV imaging is useful for visualizing and assessing NAFLD abnormalities.

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Pradipta Mukherjee, Shinichi Fukuda, Donny Lukmanto, Toshiharu Yamashita, Kosuke Okada, Shuichi Makita, Ibrahim Abd El-Sadek, Arata Miyazawa, Lida Zhu, Rion Morishita, Antonia Lichtenegger, Tetsuro Oshika, Yoshiaki Yasuno. 2022-04-18. Label-free metabolic imaging of non-alcoholic-fatty-liver-disease (NAFLD) liver by volumetric dynamic optical coherence tomography. https://doi.org/10.1364/boe.461433

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