Lensless polarimetric coded ptychography for high-resolution, high-throughput gigapixel birefringence imaging on a chip

Abstract

Polarimetric imaging provides valuable insights into the polarization state of light interacting with a sample. It can infer crucial birefringence properties of bio-specimens without using any labels, thereby facilitating the diagnosis of diseases such as cancer and osteoarthritis. In this study, we present a novel polarimetric coded ptychography (pol-CP) approach that enables high-resolution, high-throughput gigapixel birefringence imaging on a chip. Our platform deviates from traditional lens-based polarization systems by employing an integrated polarimetric coded sensor for lensless coherent diffraction imaging. Utilizing Jones calculus, we quantitatively determine the birefringence retardance and orientation information of bio-specimens from the recovered images. Our portable pol-CP prototype can resolve the 435-nm linewidth on the resolution target and the imaging field of view for a single acquisition is limited only by the detector size of 41^2. The prototype allows for the acquisition of gigapixel birefringence images with a 180-mm^2 field of view in ~3.5 minutes, a performance that rivals high-end whole slide scanner but a small fraction of the cost. To demonstrate its biomedical applications, we perform high-throughput imaging of malaria-infected blood smears, locating parasites using birefringence contrast. We also generate birefringence maps of label-free thyroid smears to identify thyroid follicles. Notably, the recovered birefringence maps emphasize the same regions as autofluorescence images, underscoring the potential for rapid on-site evaluation of label-free biopsies. Our approach provides a turnkey and portable solution for lensless polarimetric analysis on a chip, with promising applications in disease diagnosis, crystal screening, and label-free chemical imaging, particularly in resource-constrained environments.