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arXiv · 2310.11156

Enhancement of cell membrane poration by the antimicrobial peptide Melp5

Qixuan Li·Xiaoshuang Zhong·Liang Sun·Liang Dai
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Abstract

Melittin, a natural antimicrobial peptide comprising 26 amino acid residues, can kill bacteria by inducing pores in cell membranes. Clinical applications of melittin as an antibiotic require a thorough understanding of its poration mechanism and mutations that enhance its antimicrobial activity. Previous experiments showed Melp5, a variant of melittin with five mutations, exhibits a higher poration ability. However, the mechanism of the enhanced poration ability is not fully understood. Here, we investigated the mechanism by comparing the poration of melittin and Melp5 using coarse-grained (CG) and all-atom (AA) molecular dynamics (MD) simulations. We observe that Melp5 is likely to form a pore with 5 peptides (pentameric), while melittin is likely to form a pore with 4 peptides (tetrameric). Our atomistic MD simulations show that the pentameric pore of Melp5 has a higher water permeability than the tetrameric pore of melittin. We also analyze the stability of the pores of melittin and Melp5 by calculating the interaction energies of the pores. In particular, we investigate the effects of mutant residues on pore stability by calculating electrostatic and LJ interactions. These results should provide insights on the enhanced poration ability of Melp5 and push it toward applications.

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Qixuan Li, Xiaoshuang Zhong, Liang Sun, Liang Dai. 2024-01-06. Enhancement of cell membrane poration by the antimicrobial peptide Melp5. https://arxiv.org/abs/2310.11156

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