Design of an Automated Ethanol Vapor Generating System for Alcohol Use Disorder(AUD) Animal Studies

Abstract

Alcohol Use Disorder (AUD) is a prevalent addictive disorder affecting an estimated 29.5 million Americans. It is characterized by impaired control over alcohol consumption despite negative consequences. The number of diagnostic criteria met by an individual typically determines the severity of AUD. Research into AUD focuses on understanding individual susceptibility differences and developing preventive strategies. Alcohol vapor inhalation has emerged as a promising method for pathophysiological investigations in animals, allowing researchers to control the dose and duration of alcohol exposure. This approach is crucial for studying the escalation of voluntary alcohol-drinking behavior. Current commercial systems for alcohol vapor generation have limitations, including combustion risks and the need to adjust multiple parameters. Other methods, like bubbling or blow-over evaporation, face challenges in maintaining equilibrium and avoiding aerosolization. To address these issues, a new type of ethanol vapor generating system is proposed that relies solely on temperature control, creating a vacuum into which ethanol evaporates under thermodynamic control. This approach eliminates the need to adjust multiple parameters and offers improved accuracy and precision in vapor dose delivery. We validated the system as anticipated, achieving stable ethanol vapor after a few priming cycles. Using a 1.2 L cylinder, we obtained approximately 3.6 L of saturated vapor/air mix in 1 minute. Gravimetric results showed that each cycle produced about 100 mg/L or ~10,000 ppm vapor-to-air mixture. The intended use of the ethanol vapor generator is to provide a concentrated ethanol vapor / air mixture to be further diluted before delivering to the animals.