Diffusing Alpha-emitters Radiation Therapy: In vivo Measurements of Effective Diffusion and Clearance Rates Across Multiple Tumor Types

Abstract

Diffusing alpha-emitters radiation therapy (Alpha-DaRT) is a new modality that uses alpha particles to treat solid tumors. Alpha-DaRT employs interstitial sources loaded with low activities of Radium-224, which release a chain of short-lived alpha-emitters diffusing over a few millimeters around each source. Alpha-DaRT dosimetry is described, to first order, by a framework called the diffusion-leakage (DL) model. The aim of this work is to estimate the tumor-specific parameters of the DL model from in-vivo studies on multiple histological cancer types. Autoradiography studies with phosphor imaging were conducted on 113 mice-borne tumors from 10 cancer cell lines. An observable, referred to as the effective diffusion length, Leff, was extracted from images of histological slices obtained using phosphor screens. The tumor and Alpha-DaRT source activities were measured after excision with a gamma counter to estimate the probability of Lead-212 clearance from the tumor by the blood, Pleak(Pb). The measured values of Leff are in the range of 0.2-0.7 mm across different tumor types and sizes. Pleak(Pb) is between 10% and 90% for all measured tumors, and it generally decreases in magnitude and spread for larger tumors. The measured values of Leff and Pleak(Pb) and associated dose calculations indicate that hexagonal Alpha-DaRT source lattices of ~4 mm spacing with muCi-scale Radium-224 activities can lead to effective coverage of the tumor volume with therapeutic dose levels, with considerable margin to compensate for local variations in diffusion and leakage.