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arXiv · 2503.18000

Two-step excitation of fluorescent proteins with real intermediary states

J. David Wong-Campos·Dalia P. Ornelas-Huerta·Mackenzie Dion
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Abstract

We demonstrate sequential two-photon fluorescence microscopy using forbidden state transitions. Nonlinear red excitation leads to green fluorescence in live cells expressing eYFP, maintaining optical sectioning and allowing deep tissue imaging with simple optical systems.

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J. David Wong-Campos, Dalia P. Ornelas-Huerta, Mackenzie Dion. 2025-03-18. Two-step excitation of fluorescent proteins with real intermediary states. https://arxiv.org/abs/2503.18000

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