Mapping gene expression dynamics to developmental phenotypes with information entropy analysis

Abstract

The development of multicellular organisms entails a deep connection between time-dependent biochemical processes taking place at the subcellular level, and the resulting macroscopic phenotypes that arise in populations of up to trillions of cells. A statistical mechanics of developmental processes would help to understand how microscopic genotypes map onto macroscopic phenotypes, a general goal across biology. Here we follow this approach, hypothesizing that development should be understood as a thermodynamic transition between non-equilibrium states. We test this hypothesis in the context of the fruit fly, Drosophila melanogaster, a model organism used widely in genetics and developmental biology for over a century. Applying a variety of information-theoretic measures to public transcriptomics datasets of whole fly embryos during development, we show that the global temporal dynamics of gene expression can be understood as a process that probabilistically guides embryonic dynamics across macroscopic phenotypic stages. In particular, we demonstrate signatures of irreversibility in the information complexity of transcriptomic dynamics, as measured mainly by the permutation entropy of indexed ensembles (PI entropy). Our results show that the dynamics of PI entropy correlate strongly with developmental stages. Overall, this is a test case in applying information complexity analysis to relate the statistical mechanics of biomarkers to macroscopic developmental dynamics.