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arXiv · 2609.14162

Magnetic Particle Spectroscopy for Detecting Cell-Associated Zinc Ferrite Nanoparticles and Probing Their Relaxation Dynamics

Hanlei Wang·Bahareh Rezaei·Md Shahriar·Changxue Xu·Rui He·Kai Wu
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Abstract

Magnetic particle spectroscopy (MPS) enables sensitive detection of magnetic nanoparticles (MNPs) and characterization of their dynamic magnetization through higher-order harmonics. Here, we synthesized citrate-functionalized, 30 nm cubic Zn0.4Fe2.6O4 (ZFO) MNPs and investigated their association with SKOV3 ovarian cancer cells using MPS. The ZFO MNPs exhibited a crystalline spinel structure, a mean hydrodynamic diameter of 39.4 nm, strong room-temperature magnetization, low coercivity, and citrate-associated surface functional groups. Live/Dead imaging indicated good cytocompatibility after 24 h exposure at concentrations up to 0.5 mg/mL, while bright-field microscopy showed concentration-dependent cell-associated nanoparticle accumulation. Two MPS drive fields were compared using ZFO MNPs dispersed in DI water and agar as relatively unrestricted and strongly confined reference states. The 7.75 kHz, 20 mT condition retained more higher-order harmonics than 11.37 kHz and 10 mT and produced a larger and order-dependent spectral separation between the two states; it was therefore selected for the cellular measurements. After ZFO exposure and removal of unbound nanoparticles, MPS detected cell-associated MNPs in samples containing 0.1, 1, and 2x10^6 SKOV3 cells. The 3rd-, 5th-, and 7th-harmonic amplitudes increased proportionally with cell number, with power-law exponents of 1.03-1.09. We also report that most normalized harmonic ratios from the 1- and 2x10^6-cell samples fell between the water and agar references, indicating partial restriction of Brownian rotation in the cellular environment. These findings demonstrate that MPS can detect cell-associated MNPs while providing complementary spectral information about their ensemble-averaged physical confinement, supporting its application in magnetic cell labeling and cell-tracking studies.

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Hanlei Wang, Bahareh Rezaei, Md Shahriar, Changxue Xu, Rui He, Kai Wu. 2026-09-12. Magnetic Particle Spectroscopy for Detecting Cell-Associated Zinc Ferrite Nanoparticles and Probing Their Relaxation Dynamics. https://arxiv.org/abs/2609.14162

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