arXiv2026
Motile microorganisms navigate environments that vary in space and time by coupling sensory inputs to internal physiological states and active motion. In photosynthetic microorganisms, such coupling can be mediated by phototaxis, yet how temporal sensing combines with spatial variations of the environment to shape population organisation remains poorly understood. To probe this coupling, we expose populations of the phototactic microalga \textit{Chlamydomonas reinhardtii} to structured light landscapes. Under ring-shaped illumination, cells robustly accumulate at the center of the illuminated region, rather than at the light-intensity maximum. Single-cell tracking shows that cells steer relative to local intensity gradients and undergo a non-trivial phototactic response, where, on average, cells outside the ring perform positive phototaxis while cells inside perform negative phototaxis. Population experiments, numerical simulations and analytical modelling show that this trapping cannot be reproduced by an instantaneous switching rule, but requires a finite phototactic memory acting on the transition between positive and negative phototaxis. This internal timescale couples to the geometry of the light field and to the persistence of active motion, enabling controllable spatial localisation without physical confinement. Our results generically show how memory in a single-cell sensory response can control population-level organisation in a living active system.