Separation of long DNA chains using non-uniform electric field: a numerical study

Abstract

We study migration of DNA molecules through a microchannel with a series of electric traps controlled by an ac electric field. We describe the motion of DNA based on Brownian dynamics simulations of a beads-spring chain. Our simulation demonstrates that the chain captured by an electrode escapes from the binding electric field due to thermal fluctuation. We find that the mobility of chain would depend on the chain length; the mobility sharply increases when the length of a chain exceeds a critical value, which is strongly affected by the amplitude of the applied ac field. Thus we can adjust the length regime, in which this microchannel well separates DNA molecules, without changing the structure of the channel. We also present a theoretical insight into the relation between the critical chain length and the field amplitude.