arXiv subjects

Mustafa Sen

Publications and source records attributed to Mustafa Sen.

3 recordsLinked to original sources

arXiv2018

Development of Carbon-Fiber Electrodes Modified for Electrochemical ATP Detection

Adenosine triphosphate (ATP) is a highly energetic molecule used as a coenzyme in the transfer of chemical energy required for the continuation of cellular metabolism. In addition, extracellular ATP has an important role in cell-to-cell communication in immune, nervous and vascular systems. ATP is detected mainly using commercially available luciferin-luciferase kits and this method does not allow local measurement. In this study, first carbon-fiber microelectrodes capable of local detection were fabricated and then, the surface of these electrodes were modified with enzymes for detection of non-electroactive ATP molecules.

physics.med-ph↗

arXiv2018

Fabrication and characterization of pH responsive nanoprobes based on ion current rectification

In this study, we investigated the ionic current rectification of glass nanopipettes modified with bovine serum albumin - glutaraldehyde (BSA-GA) artificial membrane using solutions with various pHs. Ionic current rectification is a phenomenon that is observed with nanopores as asymmetric I-V curves, where the ionic currents recorded through a nanopore differ at the same magnitude of applied electrical potentials biased with opposite polarities. The results clearly showed that modifying the tip of a nanopipette results in a pH dependent ionic current behavior. The proposed strategy is a facile method for fabrication of a pH responsive nanoprobe that has a potential for intracellular pH measurement.

physics.chem-ph↗

arXiv2017

Using electropolymerization based doping for electro-addressable functionalization of a multi-electrode array probe for nucleic acid detection

Here, we report a facile method for electro-addressable functionalization of a probe comprising of closely spaced three individually addressable carbon fiber electrodes for detection of nucleic acids. First, a multi electrode array probe comprising three adjacent carbon fiber electrodes was fabricated through pulling a three-barrel glass capillary with a single carbon fiber in each barrel using a micropuller. Second, electropolymerization based doping was used for electro-addressable functionalization of the multi-electrode array probe. To demonstrate that the current strategy works, anti-miR-34a was electrografted on only one of three electrodes by electropolymerization of pyrrole on the specific electrode. A second electrode was coated only with polypyrrole (PPy) and the third was left unmodified. Electrochemical impedance spectroscopy (EIS) was used for analysis and the results clearly showed charge transfer resistance of the PPy + anti-miR-34a modified electrode increased significantly after hybridization, while charge transfer resistance of the other two electrodes remained almost constant. The results demonstrate that the present strategy has great potential for constructing multiplex nucleic acid micro/nano biosensors for local and in situ detection of multiple nucleic acid molecules such as miRNAs at a time.

physics.ins-det↗