Nanodiamonds with silicon vacancy defects for non-toxic photostable fluorescent labeling of neural precursor cells

Tobias D. Merson; Stefania Castelletto; Igor Aharonovich; Alisa Turbic; Trevor J. Kilpatrick; Ann M. Turnley

doi:10.1364/ol.38.004170

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arXiv · 1309.5179

Nanodiamonds with silicon vacancy defects for non-toxic photostable fluorescent labeling of neural precursor cells

Tobias D. Merson·Stefania Castelletto·Igor Aharonovich·Alisa Turbic·Trevor J. Kilpatrick·Ann M. Turnley

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Abstract

Nanodiamonds (NDs) containing silicon vacancy (SiV) defects were evaluated as a potential biomarker for the labeling and fluorescent imaging of neural precursor cells (NPCs). SiV-containing NDs were synthesized using chemical vapor deposition and silicon ion implantation. Spectrally, SiV-containing NDs exhibited extremely stable fluorescence and narrow bandwidth emission with an excellent signal to noise ratio exceeding that of NDs containing nitrogen-vacancy (NV) centers. NPCs labeled with NDs exhibited normal cell viability and proliferative properties consistent with biocompatibility. We conclude that SiVcontaining NDs are a promising biomedical research tool for cellular labeling and optical imaging in stem cell research.

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Tobias D. Merson, Stefania Castelletto, Igor Aharonovich, Alisa Turbic, Trevor J. Kilpatrick, Ann M. Turnley. 2013-09-20. Nanodiamonds with silicon vacancy defects for non-toxic photostable fluorescent labeling of neural precursor cells. https://doi.org/10.1364/ol.38.004170

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